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KMID : 1145120190170010025
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Asian Journal of Beauty and Cosmetology
2019 Volume.17 No. 1 p.25 ~ p.36
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Inhibitory Effects of Three Dimensional Adipose Tissue-Derived Mesenchymal Stem Cell Conditioned Medium on Immune Response and Efficacy Evaluation of its Cream
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Kim Kyung-Hye
Lee Sun-Ray
Park Hyun-Sook
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Abstract
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Purpose: This thesis aims to confirm the skin-smoothing effects of 3D adiposetissue derived mesenchymal stem cell conditioned medium (3D-ADMSC-CM) and study how 3D-ADMSC-CM facilitates skin stimulation recovery.
Method: Since confirming that 3D-ADMSC-CM inhibits cell growth and immune reactions, we have continued to evaluate the effects of cream containing 3D-ADMSC-CM on human skin.
Results: 3D-ADMSC-CM showed superior self-replication and cell growth rate (at 1, 3 and 5days), and the presence of interleukin 1 alpha (IL-1α) was reduced by 1.4 times, compared to LPS treated. We then applied 3D-ADMSC-CM to human skin. The skin irritation improvement and sedative effects by external stimuli (chemical stimulation) showed a decrease of 25.88% in the applied area and 22.20% in the non-dermal area after 3 weeks of use compared with before the test substance use (after SLS stimulation). Skin improvement due to the external stimulation (chemical) and soothing has been reduced after 3 weeks after use up to 25.88% of applied part and 22.20% of non-applied part compared to test material before use. The skin irritation sedative effect by physical stimulation (friction) immediately after one use showed 12.53% reduction of applied area and 8.71% reduction of non-applied part. Skin roughness was reduced after 3 weeks after use up to 15.91% of applied part and 3.16% of non-applied part compared to test material before use. The final results suggested improved keratin excitation and exfoliation has been reduced after 3 weeks after use up to 78.28% of applied part and 12.26% of non-applied part compared to test material before use.
Conclusion: 3D-ADMSC-CM is effective and provides adequate skin stimulation. Cream contained 3D-ADMSC-CM soothed skin irritation improved the overall tone and texture of skin, and recovery of exfoliation by skin stimulation (chemical & physical stimulation).
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KEYWORD
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Stem cell conditioned medium, Cosmetics, Skin irritation, Soothing, Efficacy evaluation
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